Here we show that drebrin, an F-actin-associated protein, binds directly to the microtubule-binding protein EB3. In growth cones, this interaction occurs specifically when drebrin is located on F-actin in the proximal region of filopodia and when EB3 is located at the tips of microtubules invading filopodia. When this interaction is disrupted, the formation of growth cones and the extension of neurites are impaired. We conclude that drebrin targets EB3 to coordinate F-actin-microtubule. The immunogen used to raise the anti-EB3 antibody [KT36] (ab53360) was of the recombinantly expressed full length human EB3 protein. This shares 99% homology with the rat and mouse protein. We would therefore predict that the antibody would cross react. However, as wehave not tested this ourselves as yet we would not be able to guarantee this MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3) Promoter CMV Tag / Fusion Protein. mCherry (C terminal on backbone) Cloning Information Cloning method Unknown 5′ sequencing primer CMV-F 3′ sequencing primer SV40pA- Symbol, synonyms, gene family about EB3 and general information of EB3 protein, EB3 gene, et al
The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 stability during mitosis. Human recombinant EB3 is a full length protein with an N-terminal His-tag (34 kDa). The protein.
Here we provide evidence that the MT plus-end-binding (EB) proteins EB1 and EB3 have a role in the AIS in addition to their MT plus-end tracking protein behavior in other neuronal compartments. In mature neurons, EB3 is concentrated and stabilized in the AIS. We identified a direct interaction between EB3/EB1 and the AIS scaffold protein ankyrin G (ankG). In addition, EB3 and EB1 participate in AIS maintenance, and AIS disassembly through ankG knockdown leads to cell-wide up-regulation of. In neurons with this mutation, axon initial segment (AIS) plasticity is impaired by the abnormal accumulation of end-binding protein 3 (EB3) in the AIS submembrane region. IP3K-A binds to EB3 and their binding affinity is precisely regulated by protein kinase A (PKA)-dependent phosphorylation of IP3K-A at Ser119 (pSer119). The complex of IP3K-A and EB3 dissociates and reassociates rapidly during chemically induced LTP Microtubule-associated protein RP/EB family member 3 is a protein that in humans is encoded by the MAPRE3 gene. The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end. eb3, end binding protein-3, eb3-yfp, eb2-eb3, mapre3, gfp-eb3, mt-eb3, end-binding protein 3: Sentences in this view contain interactions of eb3. Help Sentence. Journal Title. Year. Mm: Mm. We find that depletion of EB3 reduces the LY6E tubularization upon WNV infection (XREF_FIG). Proc. Natl. Acad. Sci. U.S.A., 2018. 2018; Mm: Mm. We overexpressed the GFP fused tip protein EB3, which. Other names include: Microtubule-associated protein RP/EB family member 3; EB1 protein family member 3; EBF3; End-binding protein 3; RP3; MAPRE3. EB3 has a mass of 31.98kDa and an amino acid length of 281. We offer 3 antibodies to EB3, raised in Rabbit and Goat, which are suitable for Western Blot and ELISA with samples derived from Human, Mouse and Rat. Search Filters. Product Types. Primary. End-binding proteins (EBs) are the core components of microtubule plus end tracking protein complexes, but it is cur-rently unknown whether they are essential for mammalian microtubule organization. Here, by using CRISPR/Cas9- mediated knockout technology, we generated stable cell lines lacking EB2 and EB3 and the C-terminal partner-bindin
These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics EB3 Antibody (KT36) is a monoclonal anti-EB3 antibody that detects m, r, and h EB3 by WB, IP, IF and ELISA. Cited in 3 publications. Choose a Store Santa cruz biotechnology. Santa Cruz Animal Health. Choose a Language English Français 日本語 中文 한글 Español Italiano Deutsch Svenska Português. Choose a Currency US Dollars Euro Swiss Francs British Pounds Australian Dollars Canadian. End-binding protein 3 (also known as EB3 or Microtubule-associated protein RP/EB family member 3) is a 282 amino acid long protein that is 53% homologous to EB1, which is known to associate with adenomatous polyposis coli tumor suppressor. EB3 has been shown to prefer brain tissue. EB3 is highly expressed during myogenic differentiation and the loss of EB3 activity leads to the inability of.
., 2009) Here, we demonstrate that microtubule-associated end-binding protein 3 (EB3), a core component of the microtubule plus-end protein complex, binds to inositol 1,4,5-trisphosphate receptors (IP 3 Rs) through an S/TxIP EB-binding motif EB3 is a gene encoded by the symbol MAPRE3. Other names include: Microtubule-associated protein RP/EB family member 3; EB1 protein family member 3; EBF3; End-binding protein 3; RP3; MAPRE3. EB3 has a mass of 31.98kDa and an amino acid length of 281 MAPRE3 / EB3 Protein LS-G968 is a Recombinant Human MAPRE3 / EB3 produced in E. coli with His tag(s)
Finally, the finding that only EB3, but not EB1, is phosphorylated on S176 throughout mitosis by Aurora A and B (Ban et al., 2009) might regulate the functional state of EB3 and/or its affinity to MT plus ends, causing a direct impact on MT dynamics or control EB3 association with other proteins. In the latter case, there are two interesting candidates: the MT depolymerizing protein MCAK and. . (A) Western blot analysis of the cell extracts prepared from 1) cells transfected with pECFP-C1 or cells transfected with hairpin constructs encoding RNAi to 2) Luciferase; 3) EB1; 4) EB2; 5) EB3; 6) EB1+EB3; 7) EB1+EB2+EB3. EB proteins were detected by rat mAbs KT51 (anti-EB1), KT52 (anti-EB2), and KT53 (anti-EB3; see also Supplementary Figure S1). Note that anti-EB3 antibody also. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPR E3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play. pmKate2-EB3 is a mammalian expression vector encoding mKate2-EB3 fusion protein. The vector can be used for fluorescent labeling of EB3 protein in living cells. mKate2 codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al. 1996]. Human microtubule-associated EB3 protein is fused to the mKate2 N-terminus. pmKate2-EB3 vector can be used as a source of mKate2-EB3. MAPRE3 (Microtubule Associated Protein RP/EB Family Member 3) is a Protein Coding gene. Diseases associated with MAPRE3 include Neuronopathy, Distal Hereditary Motor, Type Viib and Distal Hereditary Motor Neuronopathy Type 7 . Among its related pathways are Remodeling of Adherens Junctions and PAK Pathway
Rabbit anti-end-binding protein 3 (EB3) polyclonal antibody was described previously. 13 Mouse anti-p150 Glued monoclonal antibody was obtained from Becton Dickinson Biosciences (Franklin Lakes, NJ). Affinity-purified rabbit anti-dynactin p50 (UP1097) and anti-dynein intermediate chain polyclonal antibodies were a gift from E. Holzbauer (University of Pennsylvania, Philadelphia, PA. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPγS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP. The EB3 protein level increased in late S and G 2 phase, peaked in mitosis, and then decreased into the subsequent G 1 phase (Fig. 3B). This expression pattern distinguishes EB3 from other EB1 family members, because the level of EB1 and EB2 showed no significant changes during the cell cycle (Fig. 3, A and B). In addition, the mRNA level of EB3 was not significantly altered (Fig. 3B). Thus. Microtubule end-binding (EB) proteins influence microtubule dynamic instability, a process that is essential for microtubule reorganisation during apico-basal epithelial differentiation. Here, we establish for the first time that expression of EB2, but not that of EB1, is crucial for initial microtubule reorganisation during apico-basal epithelial differentiation, and that EB2 downregulation.
EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis EB3 End binding protein 3 EG 6-OMe Ethylenglykol-6 -O -Mercaptan EZM Extrazelluläre Matrix FAK Focal-adhesion -kinase FN Fibronektin GAP GTPase activating protein GDI Guanine nucleotide dissociation inhibitors GEF Guanine exchange factor ICAP -1 Integrin cytoplasmic domain-associated protein 1 IgG Immunglobulin G Antikörper IgM Immunglobulin M Antikörper ILK Integrin -linked -kinase mkl. Highlights 100% Casein Complex von Scitec Nutrition Leckerer Geschmack Laktosearm Gute Bekömmlichkeit Verschiedene Geschmacksrichtungen Auch als Vorratspack erhältlich Das 100% Casein wird aus reinem Casein Milchproteinen... Inhalt 0.92 Kilogramm (21,63 € * / 1 Kilogramm) 19,90 € *. Vergleichen. Jetzt bestellen EB3 is a protein that binds to the plus-ends of microtubules and can be visualized tracking through the cytoplasm. Video 1 - Run Time: 20 Seconds - DsRed-labeled mitochondria are visible moving along the microtubule network, highlighted with EGFP tagged EB3. Choose a playback version: Streaming Video (4.8 MB), Progressive Download (4.8 MB), and MPEG Download (71.8 MB) Video 2 - Run Time: 6. microtubule associated protein RP/EB family member 3 , EB3 , EBF3 , EBF3-S , RP3 Cellular Localization Cytoplasm, cytoskeleton Background The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq] Database Gene ID.
Reiko Ban, Hideki Matsuzaki, Tomohiro Akashi, Gyosuke Sakashita, Hisaaki Taniguchi, Sam-Yong Park, Hirofumi Tanaka, Koichi Furukawa, Takeshi Uran The end-binding protein 1, 2 and 3 (EB1-3) are involved in microtubule polymerization and stabilization with centrosomes as well as spindle function. The N-terminal domain of EB proteins binds to microtubules whereas the C-terminal domain forms mutually exclusive complexes with APC and DCTN1. This antibody recognizes the human EB3 in ELISA, Western blot and immunoprecipitation
microtubule-associated protein, RP/EB family, member 3, EB1 protein family member 3, microtubule-associated protein RP/EB family member 3, End-binding protein 3, APC binding protein. Immunogen. GST-tagged recombinant protein corresponding to human EB3. Protein Name EB3 is a protein that binds to the plus-ends of microtubules and can be visualized tracking through the cytoplasm. Fox lung cells with fluorescent proteins in the mitochondria and Golgi complex - Due to its relatively large size, the Golgi apparatus was one of the first organelles ever observed Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein) May 2003 The Journal of Neuroscience : The Official Journal of the.
EB3-GFP displays a punctate and short linear distribtion. Immunocytochemical staining of the same field of view with a monoclonal antibody against α-tubulin (EMD Millipore Cat. No. 05-829) reveals to the typical filamentous pattern of microtubules. Overlay of EB3-GFP (green), anti-tubulin (red) and DAPI (blue) demonstrates that EB3-GFP is localized to the microtubule ends EB3 protein, mouse [MeSH concept] Semantic type(s) Amino Acid, Peptide, or Protein [Semantic type] Biologically Active Substance [Semantic type] Keyword's position in hierarchy(ies) : You can consult : All resources. or only the major. or use the search engine. recommendations. documents concerning education. documents for patients. E-mail (Nous ne répondons pas aux questions de santé.
EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. + Oligomerization Organism Molecular Weight Cofactor; Weak dimer: Aequorea victoria : 26.9 kDa-FPbase ID: R9NL8. Attributes. Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min. End-binding proteins, such as EB1 and EB3, recruit proteins to the growing tips of MTs by virtue of [ST]X[IL]P motifs. Although TACC3 has no [ST]X[IL]P motif, it was detected in a pull-down for GST-EB1 binding proteins, albeit with low confidence (Jiang et al., 2012). To test whether or not EB proteins play a role in TACC3 +TIP behavior, we visualized GFP-TACC3 in cells depleted of EB1 and EB3.
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1). SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with. REGN-EB3 is Regeneron's investigational triple antibody cocktail treatment for Ebola virus infection and is currently under Priority Review by the U.S. Food and Drug Administration (FDA), with a target action date of October 25, 2020. Contingent on FDA approval, Regeneron expects to deliver an established number of treatment doses over the course of six years and receive compensation of. Berechnung Protein/Creatinin Quotient (mg/mmol Creatinin): (U-Gesamtprotein [g/L] × 1000)/(U-Creatinin [µmol/L] / 1000) Referenzbereich: Referenzbereiche sind methodenabhängig. Massgebend sind die Angaben auf den Resultatformularen. Material. Alter. Referenzbereich. Urin. Erwachsene < 11.3 mg/mmol Creatinin . Taxpunkte, AL-Position (8.7/2.5) 11.2 Taxpunkte: 1635.00/1510.00: Indikation. EB3 protein, rat MeSH Supplementary Concept. Preferred Label: EB3 protein, rat; MeSH note: RefSeq NM_001007656; Is substance: O; Details. Origin ID: C534832; UMLS CUI: C2607754; MeSH term(s) associated for indexing. microtubule-associated proteins [MeSH Descriptor] Record concept(s) EB3 protein, rat [MeSH concept] Semantic type(s) Amino Acid, Peptide, or Protein [UMLS semantic type] Keyword's.
Sample name: EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) Proteins: Alpha tubulin, Beta tubulin, EB3 Experimental Information Detail APC binding protein; EB 3; EB1 protein family member 3; EB3; EBF 3; EBF3; EBF3 S; End binding protein 3; End-binding protein 3; MAPRE 3; MAPRE3; MARE3_HUMAN; Member 3; Microtubule Associated Protein; Microtubule associated protein RP/EB family member 3; Microtubule-associated protein RP/EB family member 3; OTTHUMP00000200910; RP 3; RP/EB Family; RP3; Applications: IF/ICC 1:100-1:500, WB 1:500. REGN-EB3 (also known as REGN3470-3471-3479) was invented by Regeneron using its VelociSuite ® technologies; the therapy combines three fully-human monoclonal antibodies. REGN-EB3 has received orphan drug designation from both the U.S. Food and Drug Administration and the European Medicines Agency. REGN-EB3 is being developed, tested and manufactured as part of an agreement established in 2015 with the Biomedical Advanced Research and Development Authority (BARDA), part of the Office of the.
. (A) Prophase: (1) Chromosomes condense and become visible; (2) centrosomes move apart toward opposite poles and generate new microtubules. We found a strong correlation between increasing amounts of overexpressed EB1 or EB3 proteins and an increase in full length human tau levels in the culture medium (Fig 4 and S1 Fig), suggesting that EBs might be involved in the secretion of tau protein to the extracellular milieu. Download: PPT PowerPoint slide PNG larger image TIFF original image Fig 4. Overexpression of EB proteins. EB3 polyclonal antibody Catalog: BS60423 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigene-sis of colorectal cancers and proliferative control of nor-mal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade down-stream of the TCR.
GTSE1 is an EB1-dependent Microtubule Plus End Growth Tracking Protein. We previously described GTSE1 as localizing coincident with interphase microtubule networks in antibody stainings -, and in a yeast 2-hyrid screen for GTSE1-interacting proteins, EB1 emerged as a strong candidate.To investigate more closely potential microtubule- and EB1- related functions of GTSE1, we tagged the GTSE1. Here we show that CLIP-170 and a closely related protein CLIP-115 bind directly to EB1 and EB3 while displaying a lower afﬁnity for EB2. This interaction depends on the C-terminal tails of the EB proteins, which are strikingly similar to those of -tubulin. Further, we demonstrate that simulta- neous depletion of EB1 and EB3 in cultured cells causes a reduced accumulation of CLIPs at the MT. Epidermolysis bullosa (EB) ist eine genetisch bedingte Hautkrankheit, die je nach Subtyp autosomal-dominant oder -rezessiv vererbt wird. Betroffene werden als Schmetterlingskinder bezeichnet, weil ihre Haut so verletzlich wie die Flügel eines Schmetterlings sei. Ursache ist eine angeborene Mutation in bestimmten Genen, deren Genprodukte u. a. für den intakten zellulären Aufbau der Haut. Here we provide evidence that the F-actin-binding protein drebrin binds directly and specifically to the +TIP protein EB3, but not to EB1, a closely related family member that is also a +TIP protein . In growth cones, the interaction between drebrin and EB3 occurs in the proximal region of filopodia when dynamic microtubules enter the filopodium and become aligned alongside the bundle of F. As mentioned above, tau interacts with other microtubular proteins, such as the EB1 and EB3 proteins (7), in developing neuronal cells. Characterization of tau binding to EBs is currently under study. Also, tau can interact with other proteins, like the RNA binding protein TIA1, through a RNA intermediate (Vanderweyde et al., 2016)
Microtubule-associated end-binding protein 3 (EB3) accumulates asymmetrically at the tip-end of growing microtubules, providing a central platform for linking various cellular components. EB3 orchestrates microtubule dynamics and targeting, enabling diverse processes within neurons. Inositol 1, 4, 5-trisphosphate 3-kinase A (IP3K-A; also known as ITPKA) is a neuron-enriched protein that binds. Here, we find that a cell-cell competition mediated by a trans-synaptic organizing protein, eB3, can control the relative distribution of synaptic contacts between neighboring neurons, without altering the overall number of synapses . By generating a trans-cellular competition, eB3 may act as a cue that enables neurons to 'interview' potential synaptic partners with appropriate pre. Alleleustrious pmTFP1-EB3 is a mammalian expression vector that encodes a fusion of human EB3 N-terminal to mTFP1. EB3, a ~31kD protein, is also known as microtubule-associated protein, RP/EB family, member 3 (MAPRE3), and APC binding protein. This product is a great marker for cytoplasmic microtubules . Genes Dev. 2005 Oct 15;19(20):2501-15. PMID: 16230537 PubMed. Komarova Y, Lansbergen G, Galjart N, Grosveld F, Borisy GG, Akhmanova A. EB1 and EB3 control CLIP dissociation from the ends of growing microtubules. Mol.